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OCR: ng a basal In the 'short' tetracosactrin test, after tak plasma cortisol sample, 250ug tetracosa nuscularly (Synacthen) is administered either intra rtisol assay or intravenously. Further samples for co wing the are taken at thirty and sixty minutes fol 00 and the injection. This test is best performed at patient does not need to be fasting. 1 is greater The normal increment in plasma cortis than 200nmol/1 (Fig. 9.8).